In this study a qualitative and quantitative analysis of flavonoids from flower heads of Achillea species belonging to the A. millefolium L. group was achieved. Rutin, apigenin-7-O-glucoside, luteolin-7-O-glucoside in the methanolic extract of A. collina J. Becker ex Reichenb., A. pratensis Saukel et Langer, A. millefolium L. were determined by comparison of their Rf-TLC data with those obtained from reference flavonoids. Apart from flavonoid glycosides, the free aglycones, apigenin and luteolin were identified by TLC. Flavonoidic compounds have been detected by spectrophotometric method in concentrations of appr. 0.5-1.8% in plant materials.