A study was carried out to determine the source rate from a nuclear power plant using the tracer experimental data conducted at the Yeoung-Kwang nuclear site. The least squares method optimises the agreement between the released source rate and the calculated source rate by minimising the errors between the measured concentrations and the calculated ones using the Gaussian plume model. The least squares estimator generally estimates the source rate to be within a factor of 2. The forecasting ability of the source rate is improved by applying the modified dispersion coefficients that are calculated using the experimental data. Determination of the source rate in an early phase nuclear emergency will be helpful for the decision making when taking appropriate and prompt countermeasures in the case of a radiological emergency.