Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

Rapid Commun Mass Spectrom. 2005;19(5):605-10. doi: 10.1002/rcm.1831.

Abstract

Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adsorption
  • Coated Materials, Biocompatible / analysis
  • Coated Materials, Biocompatible / chemistry*
  • Electric Conductivity
  • Gold / chemistry*
  • Membranes, Artificial*
  • Peptide Mapping / methods
  • Protein Binding
  • Proteins / analysis*
  • Proteins / chemistry*
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Coated Materials, Biocompatible
  • Membranes, Artificial
  • Proteins
  • Gold