Abstract
Recently, we found that the MeOH extract of Penicillium oxalicum showed inhibitory activity towards DNA topoisomerase I. Subsequently, ergosterol peroxide, ergosterol, palmitoleic acid, and linoleic acid were isolated from the cultured mycelia of P. oxalicum. The structural determinations were based on physical and spectral analyses. Biological evaluation revealed that ergosterol peroxide inhibited the relaxation of supercoiled DNA (pBR322) induced by DNA topoisomerase I, and also showed marginal, selective cytotoxic activity against human colon tumor cells [COLO-205 (ED50=8.56 microg/mL].
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aromatase Inhibitors / administration & dosage
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Aromatase Inhibitors / pharmacology*
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Aromatase Inhibitors / therapeutic use
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DNA Topoisomerases, Type I / drug effects
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Ergosterol / administration & dosage
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Ergosterol / analogs & derivatives*
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Ergosterol / pharmacology*
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Ergosterol / therapeutic use
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Humans
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Penicillium*
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use
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Topoisomerase I Inhibitors*
Substances
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Aromatase Inhibitors
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Plant Extracts
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Topoisomerase I Inhibitors
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DNA Topoisomerases, Type I
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ergosterol-5,8-peroxide
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Ergosterol