Abstract
The mechanism of action, leakage of cytochrome c from mitochondria into cytosol, for the antineoplastic compound glyfoline was examined. Additionally, our current studies revealed that glyfoline induced apoptotic changes and arrested cell cycle procession at the G2/M phase in nasopharyngeal carcinoma (NPC). A reverse transcriptase polymerase chain reaction (RT-PCR) showed no specific changes of apoptosis-related gene expression (i. e., bax, ICE-alpha,beta, bcl-2, and c-myc). However, no similar changes were detected in fibroblasts and peripheral lymphocytes after glyfoline treatment suggesting that glyfoline has a higher affinity for tumor cells than for normal cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acridines / administration & dosage
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Acridines / pharmacology*
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Acridines / therapeutic use
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Antineoplastic Agents, Phytogenic / administration & dosage
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Antineoplastic Agents, Phytogenic / pharmacology*
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Antineoplastic Agents, Phytogenic / therapeutic use
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Apoptosis / drug effects
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Apoptosis / genetics
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Cell Line, Tumor / drug effects
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Cell Line, Tumor / metabolism
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Cytochromes c / drug effects*
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Cytochromes c / metabolism
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DNA, Neoplasm / analysis
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Gene Expression Regulation, Neoplastic
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Humans
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Inhibitory Concentration 50
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Mitochondria / drug effects
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Mitochondria / metabolism
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Nasopharyngeal Neoplasms / genetics
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Nasopharyngeal Neoplasms / pathology
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use
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Reverse Transcriptase Polymerase Chain Reaction
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Rutaceae*
Substances
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Acridines
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Antineoplastic Agents, Phytogenic
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DNA, Neoplasm
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Plant Extracts
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glyfoline
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Cytochromes c