Detection of binary-toxin genes (cdtA and cdtB) among Clostridium difficile strains isolated from patients with C. difficile-associated diarrhoea (CDAD) in Poland

J Med Microbiol. 2005 Feb;54(Pt 2):143-147. doi: 10.1099/jmm.0.45799-0.

Abstract

Clostridium difficile A+ B+ and A- B+ strains isolated from stool samples of patients with C. difficile-associated diarrhoea (CDAD) were selected from the University Hospital Warsaw collection. The binary-toxin genes cdtA and cdtB were detected by PCR in five of the 41 A+ B+ strains tested, but in none of the 17 A- B+ strains tested, giving 8.6 % prevalence (5/58) of binary-toxin-positive strains. All of the strains that were positive for binary-toxin genes were grouped into toxinotype IV, suggesting that in this institution toxinotype IV might dominate among the population of C. difficile with binary-toxin genes.

MeSH terms

  • Adult
  • Bacterial Proteins / genetics*
  • Bacterial Toxins / genetics*
  • Child
  • Child, Preschool
  • Clostridioides difficile / classification
  • Clostridioides difficile / genetics*
  • Clostridioides difficile / isolation & purification
  • Diarrhea / microbiology*
  • Enterocolitis, Pseudomembranous / microbiology
  • Enterotoxins / genetics*
  • Humans
  • Poland / epidemiology

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Enterotoxins
  • tcdA protein, Clostridium difficile
  • toxB protein, Clostridium difficile