pH-dependent quenching of the fluorescence of tryptophan residues in class A beta-lactamase from E. coli (TEM-1)

Christo Christov; Dimitar Ianev; Alexandra Shosheva; Boris Atanasov

doi:10.1515/znc-2004-11-1210

pH-dependent quenching of the fluorescence of tryptophan residues in class A beta-lactamase from E. coli (TEM-1)

Z Naturforsch C J Biosci. 2004 Nov-Dec;59(11-12):824-7. doi: 10.1515/znc-2004-11-1210.

Authors

Christo Christov¹, Dimitar Ianev, Alexandra Shosheva, Boris Atanasov

Affiliation

¹ Institute of Organic Chemistry Bulgarian Academy of Science, Sofia 1113, Bulgaria. christov@sg.uji.es

PMID: 15666541
DOI: 10.1515/znc-2004-11-1210

Abstract

We performed an investigation of the pH-dependent quenching of the fluorescence of tryptophan residues of TEM-1 beta-lactamase from E. coli by uncharged and charged quenchers. pH-dependent Stern-Volmer constants (Ksv/pH) of tryptophan residues allowed us to determine subtle but discrete structurally and functionally important processes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / enzymology*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / isolation & purification
Hydrogen-Ion Concentration
Kinetics
Spectrometry, Fluorescence / methods
Tryptophan*
beta-Lactamases / chemistry*
beta-Lactamases / isolation & purification

Substances

Escherichia coli Proteins
Tryptophan
beta-Lactamases
beta-lactamase TEM-1