Fermentation of a milk-soymilk and Lycium chinense Miller mixture using a new isolate of Lactobacillus paracasei subsp. paracasei NTU101 and Bifidobacterium longum

J Ind Microbiol Biotechnol. 2004 Dec;31(12):559-64. doi: 10.1007/s10295-004-0184-z. Epub 2004 Nov 18.

Abstract

A milk-soymilk mixture was fermented using Lactobacillus paracasei subsp. paracasei NTU101 and Bifidobacterium longum BCRC11847 at different inoculum ratios (1:1, 1:2, 1:5, 2:1, and 5:1). When the inoculum ratio was 1:2, the cell numbers of both strains were balanced after 12 h of cultivation. The pH and titratable acidity were very similar at the various inoculum ratios of cultivation. The milk-soymilk mixture was supplemented with 5, 10, 15, and 20% Lycium chinense Miller juice and fermented with Lactobacillus paracasei subsp. paracasei NTU101 and B. longum BCRC11847. Sensory evaluation results showed that supplementation with 5% Lycium chinense Miller juice improved the acceptability of the fermented milk-soymilk. The fermented beverage was stored at 4 degrees C for 14 days; variations in pH and titratable acidity were slight. The cell numbers of L. paracasei subsp. paracasei NTU101 and B. longum BCRC11847 in the fermented beverage were maintained at 1.2x10(9) CFU/ml and 6.3x10(8) CFU/ml, respectively, after 14 days of storage.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bifidobacterium / metabolism*
  • Colony Count, Microbial
  • Culture Media
  • Fermentation
  • Food Microbiology
  • Hydrogen-Ion Concentration
  • Industrial Microbiology / methods
  • Lactobacillus / metabolism*
  • Lycium / metabolism*
  • Lycium / microbiology
  • Milk / metabolism*
  • Milk / microbiology
  • Probiotics*
  • Soy Milk / metabolism*

Substances

  • Culture Media