[Genetic analysis of cultivated ginseng population with the assistance of RAPD technology]

Zhongguo Zhong Yao Za Zhi. 2004 Nov;29(11):1033-6.
[Article in Chinese]

Abstract

Objective: To provide reference for breeding of cultivated ginseng by researching the genetic diversities among different strains.

Method: To make up the systematic diagram of genetic relationship by calculating the proximity coefficient with SPSS 10.0 software and clustered by between-groups linkage method.

Result: The selected 18 random primers used in PCR amplification to produce 145 bands in 17 samples, among which 53 bands (36.5%) are polymorphic. The results of cluster analysis show that the genetic relationship between different strains of Da-maya is closer than that of Er-maya, which proves that it is feasible to differentiate the cultivated groups in molecular level on outside character.

Conclusion: RAPD is a favorable molecular marker to assist ginseng breeding.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cluster Analysis
  • DNA Primers / genetics
  • DNA, Plant / genetics*
  • Genetic Variation
  • Panax / classification
  • Panax / genetics*
  • Panax / growth & development
  • Phylogeny
  • Plants, Medicinal / genetics*
  • Plants, Medicinal / growth & development
  • Random Amplified Polymorphic DNA Technique / methods

Substances

  • DNA Primers
  • DNA, Plant