Abstract
A DNA vaccine encoding the fusion (F) gene (DNA-F) of bovine respiratory syncytial virus (BRSV) induced significant protection against BRSV infection in young calves. However, serum antibody to RSV developed more slowly in animals vaccinated with DNA-F when compared with those previously infected with BRSV. Furthermore, protection against BRSV infection was not as great as that induced by prior BRSV infection. Although there was little difference in the level of protection induced in calves vaccinated with DNA-F by either the intramuscular (i.m.) or intradermal (i.d.) routes, only the i.m. route primed for a rapid BRSV-specific IgA response after BRSV challenge. These results indicate that a DNA vaccination may be effective against RSV infection even in very young infants and calves.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / analysis
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Antibodies, Viral / biosynthesis
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Antibody Specificity
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Cattle
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Cattle Diseases / immunology
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Cattle Diseases / prevention & control*
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Cell Proliferation
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Enzyme-Linked Immunosorbent Assay
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Female
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Immunoglobulin A / biosynthesis
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Immunoglobulin G / biosynthesis
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Lung / virology
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Lymphocytes / immunology
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Mice
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Mice, Inbred BALB C
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Respiratory Syncytial Virus Infections / immunology
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Respiratory Syncytial Virus Infections / prevention & control*
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Respiratory Syncytial Virus Infections / veterinary*
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Respiratory Syncytial Virus Vaccines / genetics
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Respiratory Syncytial Virus Vaccines / immunology*
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Respiratory Syncytial Virus, Bovine / genetics
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Respiratory Syncytial Virus, Bovine / immunology*
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Reverse Transcriptase Polymerase Chain Reaction
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Vaccines, DNA / immunology
Substances
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Antibodies, Viral
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Immunoglobulin A
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Immunoglobulin G
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Respiratory Syncytial Virus Vaccines
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Vaccines, DNA