Abstract
Hypoglycemic effect of Gentiana olivieri Griseb. (Gentianaceae) flowering herbs on oral administration were studied using in vivo models in normal, glucose-hyperglycemic and streptozotocin-induced diabetic rats. Through in vivo bioassay-guided fractionation processes isoorientin, a known C-glycosylflavone, was isolated from the ethylacetate fraction by silica gel column chromatography as the main active ingredient from the plant. Isoorientin exhibited significant hypoglycemic and antihyperlipidemic effects at 15 mg/kg b.w.dose. Isoorientin concentration of the extracts and fractions were determined by HPLC in order to establish a correlation between the hypoglycaemic activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Blood Glucose / analysis
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Body Weight / drug effects
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Cholesterol / blood
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Diabetes Mellitus, Experimental / drug therapy*
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Dose-Response Relationship, Drug
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Gentiana / chemistry*
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Hyperglycemia / drug therapy*
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Hypoglycemic Agents / isolation & purification
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Hypoglycemic Agents / therapeutic use*
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Hypoglycemic Agents / toxicity
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Lethal Dose 50
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Luteolin / isolation & purification
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Luteolin / therapeutic use*
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Luteolin / toxicity
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Male
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Mice
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Plant Components, Aerial / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / therapeutic use
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Plant Extracts / toxicity
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Rats
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Rats, Sprague-Dawley
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Solvents
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Triglycerides / blood
Substances
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Blood Glucose
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Hypoglycemic Agents
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Plant Extracts
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Solvents
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Triglycerides
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Cholesterol
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homoorientin
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Luteolin