The mosses on the floor of 22 sites at 20 main parks and 2 chemical plants in Shanghai city were investigated and sampled. Based on 75 recorded moss species and their coverage, Two-way Indicator Species Analysis (TWINSPAN) and Detrended Correspondence Analysis (DCA) were used to analyze their distribution patterns. The results showed that the study sites could be identified as three groups. Group 1 included two chemical plants and two parks at the center of the city, with 23 moss species and the smallest total coverage of 21.29%; group 2 included part of the parks at the center of the city and surrounding areas, with 44 moss species and total coverage of 37.94%; and group 3 mainly included the parks at surrounding areas and suburbs of the city, with 49 moss species and total coverage of 49.66%. The results reflected a certain correlation of the distribution of moss species with different habitats and polluted and disturbed environments in the city.