Research of anaerobic fermentation biohydrogen production from molasses, starch and milk wastewater in CSTR was conducted. The theoretic possibility of biohydrogen production from three kinds of organic materials and the hydrogen producing stability was also discussed. The results indicated that molasses and starch wastewaters are preferable feeds for anaerobic biohydrogen fermentation. The hydrocarbon is the optimal feed in the main three kinds of organic materials at present time. The sugars which dissolving better are preferable materials for biohydogen production than starch at present, but the starch is better than sugars for biohydrogen production in the future if the conditions could been controlled good. The niches are variety along with the different of feeds. The optimal pH values of stability operational controlling is 4.5 +/- 0.3 for sugars wastewater and 4.0 +/- 0.2 for starch wastewater. The ORP value must lower than -220 mV and the optimal value is about -300 mV. The milk wastewater is not the suitable biohydrogen production material in CST reactor.