In this article, a minimally invasive injection method to enhance the addition of cryoprotective agent into the target biological tissues or organs to be cryopreserved was studied. Compared with the traditional treatment through immersion and permeation, direct injection could be much more effective and faster. To illustrate the benefit of this approach, a mathematical model for the addition of cryoprotective agent was established and experiments were performed to test the differences given by the two methods. Both the theoretical and the experimental results demonstrate that the minimally invasive injection method for enhancing the addition of cryoprotective agent is a promising substitution for the commonly used immersing method.