Microstructured arrays with pre-synthesized capture probes for DNA detection based on metal nanoparticles and silver enhancement

J Fluoresc. 2004 Jul;14(4):369-75. doi: 10.1023/b:jofl.0000031818.39925.4c.

Abstract

DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / analysis
  • DNA / chemistry
  • DNA Probes / chemical synthesis
  • DNA Probes / chemistry*
  • Gold / chemistry*
  • Image Processing, Computer-Assisted
  • Nanostructures / chemistry*
  • Nanotechnology / methods
  • Nucleic Acid Hybridization / methods
  • Oligonucleotide Array Sequence Analysis / instrumentation
  • Oligonucleotide Array Sequence Analysis / methods*
  • Silver / chemistry*
  • Staining and Labeling / methods

Substances

  • DNA Probes
  • Silver
  • Gold
  • DNA