Abstract
DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA / analysis
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DNA / chemistry
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DNA Probes / chemical synthesis
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DNA Probes / chemistry*
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Gold / chemistry*
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Image Processing, Computer-Assisted
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Nanostructures / chemistry*
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Nanotechnology / methods
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Nucleic Acid Hybridization / methods
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Oligonucleotide Array Sequence Analysis / instrumentation
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Oligonucleotide Array Sequence Analysis / methods*
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Silver / chemistry*
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Staining and Labeling / methods
Substances
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DNA Probes
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Silver
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Gold
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DNA