A novel nested PCR for the diagnosis of calicivirus infections in the cat

Fulvio Marsilio; Barbara Di Martino; Nicola Decaro; Canio Buonavoglia

doi:10.1016/j.vetmic.2004.09.017

A novel nested PCR for the diagnosis of calicivirus infections in the cat

Vet Microbiol. 2005 Jan 5;105(1):1-7. doi: 10.1016/j.vetmic.2004.09.017. Epub 2004 Nov 19.

Authors

Fulvio Marsilio¹, Barbara Di Martino, Nicola Decaro, Canio Buonavoglia

Affiliation

¹ Department of Scienze Biomediche Comparate, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy. fmarsilio@unite.it

PMID: 15607078
DOI: 10.1016/j.vetmic.2004.09.017

Abstract

A novel nested PCR (nPCR) assay is reported on the diagnosis of the feline calicivirus (FCV) infection. The test was performed on 47 ocular and 40 pharyngeal swabs collected from 47 cats with respiratory syndrome; among the 87 samples examined, 18 ocular and 23 pharyngeal swabs were positive in nPCR. The nPCR sensitivity was compared to other diagnostic techniques such as virus isolation on cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR). The nPCR was more sensitive than the virus isolation and RT-PCR; therefore, it can be used for calicivirosis diagnosis in cats.

Publication types

Comparative Study

MeSH terms

Animals
Caliciviridae Infections / diagnosis
Caliciviridae Infections / veterinary*
Caliciviridae Infections / virology
Calicivirus, Feline / genetics*
Calicivirus, Feline / isolation & purification
Cat Diseases / diagnosis
Cat Diseases / virology*
Cats
Conjunctiva / virology
RNA, Viral / chemistry
RNA, Viral / genetics
Respiratory Mucosa / virology
Respiratory Tract Infections / diagnosis
Respiratory Tract Infections / veterinary*
Respiratory Tract Infections / virology
Reverse Transcriptase Polymerase Chain Reaction / methods
Reverse Transcriptase Polymerase Chain Reaction / veterinary*
Sensitivity and Specificity
Sequence Analysis, DNA

Substances

RNA, Viral