Mid-ultraviolet light-emitting diode detects dipicolinic acid

Appl Spectrosc. 2004 Nov;58(11):1360-3. doi: 10.1366/0003702042475556.

Abstract

Dipicolinic acid (DPA, 2,6-pyridinedicarboxylic acid) is a substance uniquely present in bacterial spores such as that from anthrax (B. anthracis). It is known that DPA can be detected by the long-lived fluorescence of its terbium chelate; the best limit of detection (LOD) reported thus far using a large benchtop gated fluorescence instrument using a pulsed Xe lamp is 2 nM. We use a novel AlGaN light-emitting diode (LED) fabricated on a sapphire substrate that has peak emission at 291 nm. Although the overlap of the emission band of this LED with the absorption band of Tb-DPA (lambda(max) doublet: 273, 279 nm) is not ideal, we demonstrate that a compact detector based on this LED and an off-the-shelf gated photodetection module can provide an LOD of 0.4 nM, thus providing a basis for convenient early warning detectors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aerosols / chemistry
  • Bacillus anthracis / chemistry
  • Bacillus anthracis / isolation & purification*
  • Luminescent Measurements
  • Picolinic Acids / analysis
  • Picolinic Acids / isolation & purification*
  • Spectrophotometry, Ultraviolet / instrumentation
  • Spectrophotometry, Ultraviolet / methods*
  • Spores, Bacterial / chemistry
  • Spores, Bacterial / isolation & purification*
  • Terbium

Substances

  • Aerosols
  • Picolinic Acids
  • Terbium
  • dipicolinic acid