Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis

Ch Reisinger; I Osprian; A Glieder; H E Schoemaker; H Griengl; H Schwab

doi:10.1007/s10529-004-3521-4

Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis

Biotechnol Lett. 2004 Nov;26(21):1675-80. doi: 10.1007/s10529-004-3521-4.

Authors

Ch Reisinger¹, I Osprian, A Glieder, H E Schoemaker, H Griengl, H Schwab

Affiliation

¹ Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz, 8010, Austria.

PMID: 15604819
DOI: 10.1007/s10529-004-3521-4

Abstract

Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli. Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding alpha-hydroxy amides and acids while retaining enantiopurity.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Amidohydrolases / chemistry
Amidohydrolases / genetics
Amidohydrolases / metabolism*
Cloning, Molecular / methods
Escherichia coli / enzymology*
Escherichia coli / genetics
Hydro-Lyases / chemistry
Hydro-Lyases / genetics
Hydro-Lyases / metabolism*
Hydrolysis
Nitriles / chemistry
Nitriles / metabolism*
Protein Engineering / methods*
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Rhodococcus / classification
Rhodococcus / enzymology*
Rhodococcus / genetics

Substances

Nitriles
Recombinant Proteins
cyanohydrin
Amidohydrolases
amidase
Hydro-Lyases
nitrile hydratase