Unexpected reaction profile observed in the synthesis of propyl laurate when using Candida rugosa lipases immobilized in microemulsions based organogels

Biotechnol Lett. 2004 Oct;26(19):1517-20. doi: 10.1023/B:BILE.0000044455.42471.bf.

Abstract

1-Propyl laurate synthesis should not be used as standard reaction test of immobilized enzymes in microemulsion-based organogels (MBGs) prepared using lecithin/1-propanol as surfactant when extremely active enzymes with high load are used. In these cases, an anomalous kinetic reaction constant value is observed over short reaction times. Such an anomalous profile is strongly dependent on the concentration of catalyst in the crude powder and, consequently, is not appreciated when either commercial or low activity lipase samples are employed.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida / enzymology*
  • Chemistry Techniques, Analytical / methods*
  • Emulsions / chemistry
  • Enzyme Activation
  • Enzyme Stability
  • Enzymes, Immobilized / chemistry*
  • Esterification
  • Gels / chemistry
  • Kinetics
  • Lauric Acids / chemical synthesis*
  • Lipase / chemistry*
  • Organic Chemicals / chemistry
  • Powders
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Surface-Active Agents / chemistry*

Substances

  • Emulsions
  • Enzymes, Immobilized
  • Gels
  • Lauric Acids
  • Organic Chemicals
  • Powders
  • Surface-Active Agents
  • Lipase