The 17beta-estradiol (17beta-E2) is a steroid sex hormone that has a profound influence on the immune cells in inducing the apoptosis of both T and B lymphocytes. In this study, mouse spleen dendritic cells (SDCs) were treated with 17beta-E2 and the numbers, endocytosis, stimulative capacity and cytokine production of SDCs were analysed. The results showed that 17beta-E2 reduced the proliferation and stimulative capacity of SDCs and increased the endocytosis of SDCs in a dose-dependent pattern. 17beta-E2 up-regulated IL-10 mRNA level in SDCs in a dose-dependent manner except for the 24 h time point. These data suggest that 17beta-E2 may regulate the physiological and pathological immune response by reducing the number and stimulation of SDCs, increasing their endocytosis and IL-10 mRNA expression at the same time.