Proteomics analysis by two-dimensional differential gel electrophoresis reveals the lack of a broad response of Neisseria meningitidis to in vitro-produced AI-2

Stephan Schauder; Lucia Penna; Adeline Ritton; Catherine Manin; Fabienne Parker; Geneviève Renauld-Mongénie

doi:10.1128/JB.187.1.392-395.2005

Proteomics analysis by two-dimensional differential gel electrophoresis reveals the lack of a broad response of Neisseria meningitidis to in vitro-produced AI-2

J Bacteriol. 2005 Jan;187(1):392-5. doi: 10.1128/JB.187.1.392-395.2005.

Authors

Stephan Schauder¹, Lucia Penna, Adeline Ritton, Catherine Manin, Fabienne Parker, Geneviève Renauld-Mongénie

Affiliation

¹ Research Department, Microbiology, Aventis Pasteur, 1541 avenue Marcel Mérieux, 69280 Marcy l'Etoile, France.

Abstract

To investigate the effect of the autoinducer AI-2 on protein expression in Neisseria meningitidis, a luxS mutant of strain MC58 was grown in the presence and absence of in vitro-produced AI-2, and differential protein expression was assessed by two-dimensional differential gel electrophoresis. N. meningitidis did not show a global response to AI-2 signaling activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / analysis*
Bacterial Proteins / physiology
Carbon-Sulfur Lyases
Electrophoresis, Gel, Two-Dimensional
Homoserine / analogs & derivatives*
Homoserine / physiology*
Lactones
Neisseria meningitidis / metabolism*
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Bacterial Proteins
Lactones
N-octanoylhomoserine lactone
Homoserine
Carbon-Sulfur Lyases
LuxS protein, Bacteria