A search for an annual variation of a daily sidereal modulation of the frequency difference between colocated 129Xe and 3He Zeeman masers sets a stringent limit on boost-dependent Lorentz and CPT violation involving the neutron, consistent with no effect at the level of 150 nHz. In the framework of the general standard-model extension, the present result provides the first clean test for the fermion sector of the symmetry of spacetime under boost transformations at a level of 10(-27) GeV.