Objective: To explore the mechanism of acute ischemia-reperfusion injury (IRI) to renal-transplant
Methods: A total of 12 Chinese Sichuan White Pigs (SWPs) (2-3 months old, 17.5 +/- 4.6 kg, with clear family background) were used. 3 pairs of them with better tissue typing were subjected to renal allotransplantation. The other SWPs were allocated to renal autotransplantation. We assessed the SWPs' kidney function, serum superoxide dismutase (SOD) and malonaldehyde (MDA) after transplantation so as to shed light on the possible mechanism of IRI and to find out good detecting methods which could monitor IRI.
Results: Serum creatinine and urea increased in 2 days of postoperation and then returned to normal in both groups except the pigs that suffered from acute tubular necrosis or acute rejection. The concentrations of serum MDA and SOD began to increase 2 days after operation (P < 0.05). More increase of serum MDA and less increase of SOD were detected in renal allotransplant groups than in renal autotransplant groups (P < 0.05). The concentration of serum SOD and MDA were correlated (r = 0.961).
Conclusion: IRI could initiate lipid peroxidation. IRI could be a single factor or be synergistic with immunologic factors to injure the transplanted organs.