Objective: To establish a human to pig xenotransplantation GVHD model to simulate the human anti pig grafts immunoreaction in vivo.
Methods: The Chinese Neijiang pigs were immunosuppressed by CTX followed by the transplant of human spleen slices into pig's peritoneal cavity. We tested the routine items of the pigs' peripheral blood regularly, monitored the chimeric level by FCM, and examined the cytotoxicities of both human anti pig and pig anti human by CML.
Result: The pigs all survived during the experimental period. The PBWBC decreased by 85% after 4 days of giving CTX. The chimeric peaks appeared on day 2 and day 14 after transplant. The cytotoxicity of human anti pig was always less than that of pig anti human, although it increased significantly after 2 weeks of transplant.
Conclusion: There might be a dynamic changeable balance of HVG, microchimerism, and GVH due to the reaction between the chimeric human cells and the pig cells in the human to pig spleen xenotransplantation model.