[Conformational changes of actin induced by strong or weak myosin subfragment-1 binding]

Tsitologiia. 2004;46(8):719-34.
[Article in Russian]

Abstract

Movements of different areas of polypeptide chains within F-actin monomers induced by S1 or pPDM-S1 binding were studied by polarized fluorimetry. Thin filaments of ghost muscle were reconstructed by adding G-actin labeled with fluorescent probes attached alternatively to different sites of actin molecule. These sites were: Cys-374 labeled with 1,5-IAEDANS, TMRIA or 5-IAF; Lys-373 labeled with NBD-Cl; Lys-113 labeled with Alexa-488; Lys-61 labeled with FITC; Gln-41 labeled with DED and Cys-10 labeled with 1,5-IAEDANS, 5-IAF or fluorescein-maleimid. In addition, we used TRITC-, FITC-falloidin and e-ADP that were located, respectively, in filament groove and interdomain cleft. The data were analysed by model-dependent and model-independent methods (see appendixes). The orientation and mobility of fluorescent probes were significantly changed when actin and myosin interacted, depending on fluorophore location and binding site of actomyosin. Strong binding of S with actin leads to 1) a decrease in the orientation of oscillators of derivatives of falloidin (TRITC-falloidin, FITC-falloidin) and actin-bound nucleotide (e-ADP); 2) an increase in the orientation of dye oscillators located in the "front' surface of the small domain (where actin is viewed in the standard orientation with subdomains 1/2 and 3/4 oriented to the right and to the left, respectively); 3) a decrease in the angles of dye oscillators located on the "back" surface of subdomain-1. In contrast, a weak binding of S1 to actin induces the opposite effects in orientation of these probes. These data suggest that during the ATP hydrolysis cycle myosin heads induce a change in actin monomer (a tilt and twisting of its small domain). Presumably, these alterations in F-actin conformation play an important role in muscle contraction.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Actins / chemistry
  • Actins / metabolism*
  • Animals
  • Binding Sites
  • Cross-Linking Reagents
  • Fluorescence Polarization
  • Fluorescent Dyes
  • Maleimides
  • Mathematics
  • Muscle Contraction*
  • Myosin Subfragments / chemistry
  • Myosin Subfragments / metabolism*
  • Protein Conformation
  • Rabbits

Substances

  • Actins
  • Cross-Linking Reagents
  • Fluorescent Dyes
  • Maleimides
  • Myosin Subfragments
  • N,N'-4-phenylenedimaleimide