Characterization of a paracetamol metabolite using on-line LC-SPE-NMR-MS and a cryogenic NMR probe

J Chromatogr A. 2004 Nov 26;1058(1-2):191-6.

Abstract

In this study, the hyphenation of LC-SPE-NMR-MS at 500 MHz was applied to the structural elucidation of a low concentrated paracetamol metabolite present in human urine. Single or multiple peak trapping of the mass detected metabolite on SPE cartridges was employed to increase the sensitivity and quality NMR measurement over the conventional LC-NMR method. After the elution of the metabolite from the SPE cartridge to the NMR flow probe using deuterated acetonitrile for initial NMR investigation, the fraction was revovered by flushing the sample out of the NMR probe head with nitrogen gas. On the recovered fraction, high resolution FT-ICR-MS measurements were conducted, giving exact mass information about the unknown metabolite. In addition, a cryogenic NMR micro probe head was used to enhance the sensitivity of the NMR measurement by a factor of 5 in order to run 2D experiments for structural elucidation of the unknown metabolite. The combination of both MS and NMR results, led unequivocally to the elucidation of the structure as the ether glucuronide of 3-methoxyparacetamol.

MeSH terms

  • Acetaminophen / metabolism*
  • Chromatography, Liquid / methods*
  • Magnetic Resonance Spectroscopy / methods*
  • Mass Spectrometry / methods*
  • Molecular Probes

Substances

  • Molecular Probes
  • Acetaminophen