Evaluation of a high-density oligonucleotide array for characterization of grlA, grlB, gyrA and gyrB mutations in fluoroquinolone resistant Staphylococcus aureus isolates

J Microbiol Methods. 2005 Feb;60(2):275-9. doi: 10.1016/j.mimet.2004.09.015.

Abstract

Using high-density oligonucleotide array technology, 30 Staphylococcus aureus strains were studied for the presence of mutations in genes involved in fluoroquinolone resistance: grlA, gyrA, grlB and gyrB. For the two most important genes, gyrA and grlA, correlation with sequencing reached 95.1%. If all genes were considered, correlation was 88.8%.

Publication types

  • Evaluation Study

MeSH terms

  • Codon
  • DNA Gyrase / genetics*
  • DNA Topoisomerase IV / genetics*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial / genetics
  • Fluoroquinolones / pharmacology*
  • Humans
  • Mutation*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction
  • Staphylococcal Infections / drug therapy
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / metabolism

Substances

  • Codon
  • DNA, Bacterial
  • Fluoroquinolones
  • DNA Topoisomerase IV
  • DNA Gyrase