Post-transcriptional effects of phorbol 12-myristate 13-acetate on transcriptome of U937 cells

Hiroshi Kitamura; Tomoko Nakagawa; Michiyo Takayama; Yayoi Kimura; Atsushi Hijikata; Osamu Ohara

doi:10.1016/j.febslet.2004.11.008

Post-transcriptional effects of phorbol 12-myristate 13-acetate on transcriptome of U937 cells

FEBS Lett. 2004 Dec 3;578(1-2):180-4. doi: 10.1016/j.febslet.2004.11.008.

Authors

Hiroshi Kitamura¹, Tomoko Nakagawa, Michiyo Takayama, Yayoi Kimura, Atsushi Hijikata, Osamu Ohara

Affiliation

¹ Laboratory for Immunogenomics, Research Center for Allergy and Immunology, The Institute of Physical and Chemical Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.

PMID: 15581638
DOI: 10.1016/j.febslet.2004.11.008

Abstract

To identify post-transcriptionally modulated genes at the translational level by phorbol 12-myristate 13-acetate (PMA), we investigated mRNA profiles in the polysomal and the cytoplasmic fractions of U937 cells before and after PMA stimulation using microarrays with 15017 oligonucleotide probes. Global comparison of the profiles showed that the cytoplasmic distribution of mRNAs was considerably modulated upon PMA stimulation. The results also indicate that PMA post-transcriptionally regulated at least 0.7% of detectable genes in U937 cells. Thus, besides transcriptional modulation by PMA, changes in the translational state of transcripts seem to play a critical role in PMA-induced differentiation of U937 cells.

MeSH terms

Gene Expression Profiling*
Gene Expression Regulation / drug effects*
Humans
Oligonucleotide Array Sequence Analysis
Polyribosomes / genetics
Polyribosomes / metabolism
RNA Processing, Post-Transcriptional*
RNA, Messenger / metabolism*
Subcellular Fractions
Tetradecanoylphorbol Acetate / pharmacology*
Transcription, Genetic*
U937 Cells

Substances

RNA, Messenger
Tetradecanoylphorbol Acetate