Abstract
The objective of this study was to find out which N-terminal segment/s of amyloid precursor protein (APP) has any neurotrophic properties, since soluble APP-alpha (sAPP-alpha) has neurotrophic effects. We investigated neurotrophic properties of eight peptide segments of N-terminal APP. The APP63-73 was able to enhance neuronal growth; augment axonal and cell body growth in human neuroblastoma cell line, SH-SY5Y. Neurotrophic effects of chronic APP63-73 treatment were assessed in vivo using streptozotocin-induced diabetes and ovariectomized rats. Thus, this study demonstrated that APP63-73 peptide has neurotrophic effects both in vivo and in vitro.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid beta-Protein Precursor / chemical synthesis
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Amyloid beta-Protein Precursor / pharmacology*
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Amyloid beta-Protein Precursor / therapeutic use
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Analysis of Variance
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Animals
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Behavior, Animal / drug effects
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Cell Count / methods
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Cell Line, Tumor
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Cell Size / drug effects
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Cell Survival / drug effects
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Diabetes Mellitus, Experimental / drug therapy
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Disease Models, Animal
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Dose-Response Relationship, Drug
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Female
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Humans
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L-Lactate Dehydrogenase / metabolism
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Male
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Maze Learning / drug effects
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Mice
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Nerve Growth Factors / chemical synthesis
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Nerve Growth Factors / pharmacology*
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Nerve Growth Factors / therapeutic use
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Neuroblastoma
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Ovariectomy / methods
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Peptide Fragments / chemical synthesis
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Peptide Fragments / pharmacology
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Peptide Fragments / therapeutic use
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Peptides / chemical synthesis
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Peptides / chemistry
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Peptides / pharmacology
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Random Allocation
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Rats
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Rats, Wistar
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Tetrazolium Salts
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Thiazoles
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Time Factors
Substances
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APP63-73
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Amyloid beta-Protein Precursor
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Nerve Growth Factors
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Peptide Fragments
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Peptides
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Tetrazolium Salts
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Thiazoles
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L-Lactate Dehydrogenase
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thiazolyl blue