Abstract
We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium / metabolism
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Cells, Cultured
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Cricetinae
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Cricetulus
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Crystallization / methods
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Endothelial Cells / cytology*
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Endothelial Cells / metabolism
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Equipment Failure Analysis
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Fiber Optic Technology
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Fura-2* / analogs & derivatives*
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Image Enhancement / instrumentation
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Image Enhancement / methods
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Lasers*
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Microscopy, Confocal / instrumentation*
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Microscopy, Confocal / methods
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Microscopy, Fluorescence, Multiphoton / instrumentation*
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Microscopy, Fluorescence, Multiphoton / methods*
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Myocytes, Cardiac / cytology*
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Myocytes, Cardiac / metabolism
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Photons
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Rats
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Reproducibility of Results
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Sensitivity and Specificity