Abstract
Kinesin-1 is a heterotetramer composed of kinesin heavy chain (KHC) and kinesin light chain (KLC). The Caenorhabditis elegans genome has a single KHC, encoded by the unc-116 gene, and two KLCs, encoded by the klc-1 and klc-2 genes. We show here that UNC-116/KHC and KLC-2 form a complex orthologous to conventional kinesin-1. KLC-2 also binds UNC-16, the C. elegans JIP3/JSAP1 JNK-signaling scaffold protein, and the UNC-14 RUN domain protein. The localization of UNC-16 and UNC-14 depends on kinesin-1 (UNC-116 and KLC-2). Furthermore, mutations in unc-16, klc-2, unc-116, and unc-14 all alter the localization of cargos containing synaptic vesicle markers. Double mutant analysis is consistent with these four genes functioning in the same pathway. Our data support a model whereby UNC-16 and UNC-14 function together as kinesin-1 cargos and regulators for the transport or localization of synaptic vesicle components.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing / chemistry
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / metabolism*
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Amino Acid Sequence
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Animals
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Blotting, Western
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans / physiology*
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Caenorhabditis elegans Proteins / chemistry
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Line
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Cytoskeletal Proteins
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Fluorescent Antibody Technique, Indirect
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Gene Expression
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Genes, Helminth
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Green Fluorescent Proteins / analysis
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Humans
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Kinesins / metabolism*
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Models, Genetic
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Molecular Sequence Data
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Mutation
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Neurons / metabolism
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Precipitin Tests
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Protein Binding
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Protein Structure, Tertiary
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Sequence Homology, Amino Acid
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Synaptic Vesicles / metabolism*
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Transgenes
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Two-Hybrid System Techniques
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Vesicular Transport Proteins / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Caenorhabditis elegans Proteins
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Carrier Proteins
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Cytoskeletal Proteins
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KLC1 protein, human
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UNC-14 protein, C elegans
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Vesicular Transport Proteins
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unc-16 protein, C elegans
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Green Fluorescent Proteins
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Kinesins