Placenta has a long history of being Chinese traditional medicine. The quality control methods used nowadays in the markets were mainly based on the amino acid content of the placenta. However, it is hard to distinguish human placenta from sheep placenta with these methods. In this paper, a novel method was established employing FT-IR and RP-HPLC techniques. This method can distinguish human placenta from sheep placenta and may help to improve quality control of placenta in the markets.