The ntrA gene of Agrobacterium tumefaciens: identification, cloning, and phenotype of a site-directed mutant

J Bacteriol. 1992 Apr;174(8):2720-3. doi: 10.1128/jb.174.8.2720-2723.1992.

Abstract

A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization and C4-dicarboxylate transport but not for vir gene expression or tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Mutagenesis, Site-Directed
  • Sigma Factor / genetics*

Substances

  • DNA, Bacterial
  • Sigma Factor