Efficient cleavage of DS DNA by bleomycin conjugated via hexaethylene glycol linker to triplex-forming oligonucleotides

Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(6-7):1047-51. doi: 10.1081/NCN-200026066.

Abstract

New conjugates of bleomycin A5 with oligonucleotides are synthesized. The bleomycin residue was attached to the 3'- or 5'- terminus of hexadecathymidilate via a hexaethylene glycol phosphate linker. Newly designed conjugates were shown to cleave site-specifically both strands of a dsDNA fragment within a triplex. The maximum extent of cleavage for individual strand amounts to 61%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography
  • Base Sequence
  • Bleomycin / chemistry*
  • DNA / chemistry*
  • Electrophoresis, Polyacrylamide Gel
  • Ethylene Glycols / chemistry*
  • Oligonucleotides / chemistry*

Substances

  • Ethylene Glycols
  • Oligonucleotides
  • Bleomycin
  • hexaethylene glycol
  • DNA