Silencing of MDR 1 gene in cancer cells by siRNA

Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(6-7):861-6. doi: 10.1081/NCN-200026032.

Abstract

Inhibition of p-glycoprotein (PGP) expression and reverse of multidrug resistance (MDR) phenotype in KB-8-5 cells by synthetic 21-bp double-stranded oligoribonucleotides were investigated. siRNA constructs for the efficient down regulation of MDR1 that are active in nanomolar concentrations and cause reversal of MDR phenotype in cells were developed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Cell Line, Tumor
  • Gene Silencing / physiology*
  • Genes, MDR*
  • Humans
  • RNA, Messenger / genetics
  • RNA, Small Interfering / physiology*
  • Rhodamine 123 / metabolism

Substances

  • RNA, Messenger
  • RNA, Small Interfering
  • Rhodamine 123