Abstract
Twenty-four calves were immunised four times with gE-deleted infectious bovine rhinotracheitis marker vaccines before being challenged with small doses of wild-type bovine herpesvirus type 1 (BHV-1). The repeated vaccinations induced strong immunity that prevented detectable virus replication and gE-seroconversion after the challenge infection in most of the calves. The hypervaccinated calves that shed virus after the challenge infection showed no delay in gE-seroconversion compared with unvaccinated control calves. Using a sensitive nested PCR, BHV-1 gE sequences could be detected in the trigeminal ganglia of several of the gE-seronegative, challenge-infected calves, possibly indicating the presence of wild-type BHV-1 DNA.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Viral / blood*
-
Cattle
-
Cattle Diseases / immunology
-
Cattle Diseases / prevention & control*
-
DNA, Viral / analysis
-
DNA, Viral / isolation & purification
-
Herpesviridae Infections / immunology
-
Herpesviridae Infections / prevention & control
-
Herpesviridae Infections / veterinary*
-
Herpesvirus 1, Bovine / immunology*
-
Polymerase Chain Reaction / methods
-
Polymerase Chain Reaction / veterinary
-
Random Allocation
-
Trigeminal Ganglion / virology
-
Vaccines, Inactivated
-
Viral Envelope Proteins
-
Viral Proteins
-
Viral Vaccines / administration & dosage
-
Viral Vaccines / immunology*
-
Virus Replication
-
Virus Shedding
Substances
-
Antibodies, Viral
-
DNA, Viral
-
Vaccines, Inactivated
-
Viral Envelope Proteins
-
Viral Proteins
-
Viral Vaccines
-
bovine herpesvirus type-1 glycoproteins