Abstract
Two xylan-degrading bacterial strains were isolated from a decayed Ulmus nigra tree in Spain. The isolates were Gram-positive, non-motile, aerobic and formed substrate mycelium which fragmented into irregular rods. 16S rRNA gene sequence analysis indicated that the isolates form a separate branch within the genus Agromyces phylogenetic cluster, with Agromyces mediolanus DSM 20152(T) being their closest relative (97.7 and 97.6 % sequence similarity). Catalase, nitrate reduction and urease tests differentiated these strains from A. mediolanus. Cell-wall peptidoglycan composition, major menaquinone, predominant fatty acids and phospholipid pattern were typical of the genus Agromyces. The DNA G+C content determined for the type strain XIL01(T) was 72 mol%. Based on the data presented, a novel species Agromyces ulmi sp. nov. is proposed. The type strain is XIL01(T) (=LMG 21954(T)=DSM 15747(T)).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetales / classification*
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Actinomycetales / cytology
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Actinomycetales / isolation & purification*
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Actinomycetales / physiology
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Aerobiosis
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Bacterial Typing Techniques
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Base Composition
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Catalase / analysis
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Cell Wall / chemistry
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / isolation & purification
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Fatty Acids / analysis
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Fatty Acids / isolation & purification
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Genes, rRNA
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Gentian Violet
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Molecular Sequence Data
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Movement
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Nitrate Reductase
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Nitrate Reductases / analysis
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Peptidoglycan / chemistry
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Peptidoglycan / isolation & purification
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Phenazines
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Phospholipids / analysis
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Phospholipids / isolation & purification
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Phylogeny
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Spain
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Ulmus / microbiology*
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Urease / analysis
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Vitamin K 2 / analysis
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Vitamin K 2 / isolation & purification
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Xylans / metabolism*
Substances
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DNA, Bacterial
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DNA, Ribosomal
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Fatty Acids
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Gram's stain
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Peptidoglycan
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Phenazines
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Phospholipids
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Xylans
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Vitamin K 2
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Catalase
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Nitrate Reductases
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Nitrate Reductase
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Urease
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Gentian Violet
Associated data
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GENBANK/AY427830
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GENBANK/AY427831