Application of microarrays in high-throughput enzymatic profiling

Mahesh Uttamchandani; Xuan Huang; Grace Y J Chen; Lay-Pheng Tan; Shao Q Yao

doi:10.1385/MB:28:3:227

Application of microarrays in high-throughput enzymatic profiling

Mol Biotechnol. 2004 Nov;28(3):227-39. doi: 10.1385/MB:28:3:227.

Authors

Mahesh Uttamchandani¹, Xuan Huang, Grace Y J Chen, Lay-Pheng Tan, Shao Q Yao

Affiliation

¹ Department of Biological Sciences, National University of Singapore, 3 Science Drive, Singapore 117543, Republic of Singapore.

PMID: 15542923
DOI: 10.1385/MB:28:3:227

Abstract

This review focuses on recent developments in microarray-based technologies for high-throughput screenings of enzymes. Novel methods of protein immobilization, detection of enzymatic activities, and inhibitions were highlighted.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Amino Acid Sequence
Enzymes / chemistry*
Enzymes / genetics
Oligonucleotide Array Sequence Analysis*

Substances

Enzymes