Abstract
Dynamic electrochemical studies, incorporating catalytic voltammetry and detailed potential-step manipulations, provide compelling evidence that the oxidized inactive state of [NiFe]-hydrogenases termed Unready (or Ni-A) contains a product of partial reduction of O(2) that is trapped in the active site.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism
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Chromatiaceae / enzymology*
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Electrochemistry
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Enzyme Activation
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Hydrogenase / chemistry*
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Hydrogenase / metabolism*
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Oxidation-Reduction
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Oxygen / chemistry*
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Oxygen / metabolism*
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Oxygen / pharmacology
Substances
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Bacterial Proteins
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nickel-iron hydrogenase
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Hydrogenase
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Oxygen