DNA-modified carbon paste electrode applied to the study of interaction between rifampicin (RIF) and DNA in solution and at the electrode surface

S Th Girousi; I Ch Gherghi; M K Karava

doi:10.1016/j.jpba.2004.08.034

DNA-modified carbon paste electrode applied to the study of interaction between rifampicin (RIF) and DNA in solution and at the electrode surface

J Pharm Biomed Anal. 2004 Nov 19;36(4):851-8. doi: 10.1016/j.jpba.2004.08.034.

Authors

S Th Girousi¹, I Ch Gherghi, M K Karava

Affiliation

¹ Analytical Chemistry Laboratory, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. girousi@chem.auth.gr

PMID: 15533679
DOI: 10.1016/j.jpba.2004.08.034

Abstract

Rifampicin (RIF) is an antibiotic widely used against tuberculosis and a DNA intercalator. The interaction of RIF with double-stranded (ds) and single-stranded (ss) calf thymus DNA was studied in solution as well as at the electrode surface by means of transfer voltammetry using differential pulse as stripping mode with carbon paste electrode (CPE) at 0.2M acetate buffer at pH 5.0 and at 0.2M phosphate buffer pH 7.4. Differentiations in the above-mentioned interaction at different pH values are presented and compared in order to optimize the detection of Rifampicin (RIF).

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon / analysis*
Cattle
DNA / analysis*
DNA / metabolism
Electrochemistry
Electrodes / standards
Rifampin / analysis*
Rifampin / chemistry
Rifampin / metabolism
Solutions

Substances

Solutions
Carbon
DNA
calf thymus DNA
Rifampin