A preliminary trial using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) on the same feedstuffs to detect tissues of animal origin

Vet Res Commun. 2004 Aug;28(6):461-6. doi: 10.1023/b:verc.0000040248.52246.82.

Abstract

A preliminary study using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) was done on the same feedstuffs to detect animal tissues. The results of the two methods differ somewhat: PCR-RFLP did not detect any signal in any sample, but multiplex PCR detected a signal in one sample. These findings could be a basis for further investigations.

Publication types

  • Evaluation Study

MeSH terms

  • Animal Feed / analysis*
  • Animals
  • Cattle
  • DNA / analysis*
  • Fishes
  • Food Contamination / analysis*
  • Goats
  • Meat / analysis
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Restriction Fragment Length*
  • Sensitivity and Specificity
  • Sheep
  • Swine
  • Turkeys

Substances

  • DNA