Abstract
Schizophyllans carrying beta-lactoside and alpha-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-beta-lactoside and alpha-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. beta-lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Count
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Circular Dichroism
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Drug Delivery Systems / instrumentation*
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Drug Delivery Systems / methods*
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Electrophoresis, Agar Gel
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Glycosides / chemistry
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Humans
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Kinetics
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Macromolecular Substances / chemical synthesis
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Macromolecular Substances / chemistry
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Oligonucleotides, Antisense / administration & dosage*
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Oligosaccharides / chemistry*
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Poly A / chemistry
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Poly C / chemistry
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Sensitivity and Specificity
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Sizofiran / chemical synthesis
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Sizofiran / chemistry*
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Surface Plasmon Resonance
Substances
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Glycosides
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Macromolecular Substances
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Oligonucleotides, Antisense
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Oligosaccharides
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lactosides
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Poly A
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poly(dA)
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Poly C
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Sizofiran