Evaluation of a colorimetric antifungal susceptibility test by using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride

Antimicrob Agents Chemother. 2004 Nov;48(11):4457-9. doi: 10.1128/AAC.48.11.4457-4459.2004.

Abstract

A colorimetric antifungal susceptibility test was performed using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride. Among 24 strains of Candida species, no trailing growth was found. In 22 and 20 strains, the MICs obtained in the colorimetric assay were within two dilutions of those obtained by the National Committee for Clinical Laboratory Standards method for ketoconazole and itraconazole, respectively.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Candida / drug effects*
  • Candida / growth & development
  • Colorimetry
  • Indicators and Reagents
  • Itraconazole / pharmacology
  • Ketoconazole / pharmacology
  • Microbial Sensitivity Tests
  • Tetrazolium Salts*

Substances

  • 2,3-diphenyl-5-thienyl-(2)-tetrazolium
  • Indicators and Reagents
  • Tetrazolium Salts
  • Itraconazole
  • Ketoconazole