Expanding the scope of site-specific protein biotinylation strategies using small molecules

Lay-Pheng Tan; Grace Y J Chen; Shao Q Yao

doi:10.1016/j.bmcl.2004.09.056

Expanding the scope of site-specific protein biotinylation strategies using small molecules

Bioorg Med Chem Lett. 2004 Dec 6;14(23):5735-8. doi: 10.1016/j.bmcl.2004.09.056.

Authors

Lay-Pheng Tan¹, Grace Y J Chen, Shao Q Yao

Affiliation

¹ Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

PMID: 15501031
DOI: 10.1016/j.bmcl.2004.09.056

Abstract

We present a new approach to site-specifically biotinylate protein in a cell-free protein synthesis system with puromycin-containing small molecules. With this new method, biotinylated proteins were generated from the DNA templates in a matter of hours, making it useful for protein microarray generation. We also validated that the method is compatible with other high-throughput cloning/proteomics methods.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites / physiology
Biotinylation / methods*
Proteins / chemical synthesis
Proteins / genetics*
Proteins / metabolism*

Substances

Proteins