[mtDNA mutations in mouse tumors]

Zhonghua Bing Li Xue Za Zhi. 2004 Oct;33(5):458-61.
[Article in Chinese]

Abstract

Objective: To investigate variations of mtDNA in mouse tumors and to explore the relationship between mtDNA mutations and murine carcinogenesis.

Methods: Variations of D-loop, ND3 and tRNAIle + Glu + Met gene fragments of mtDNA from six mouse tumor cell lines were analyzed by PCR-RFLP and PCR-SSCP techniques.

Results: ND3 and tRNAIle + Glu + Met gene fragments of mtDNA from the tumors showed no variations at 27 endonuclease sites. The D-loop of mtDNA from Hca-F demonstrated an additional endonuclease site of Hinf I in contrast to the inbred mouse. Upon PCR-SSCP analysis, the D-loop of mtDNA was found to possess mutations in 4 of 6 tumors.

Conclusion: D-loop appears to be the hot spot for tumor mtDNA mutations, which may contribute to the carcinogenesis of murine tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • DNA, Mitochondrial / genetics*
  • DNA, Neoplasm / genetics*
  • Electron Transport Complex I
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mutation*
  • Neoplasms, Experimental / genetics*
  • Neoplasms, Experimental / pathology
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single-Stranded Conformational
  • Proteins / genetics
  • RNA, Transfer, Glu / genetics
  • RNA, Transfer, Ile / genetics
  • RNA, Transfer, Met / genetics

Substances

  • DNA, Mitochondrial
  • DNA, Neoplasm
  • Proteins
  • RNA, Transfer, Glu
  • RNA, Transfer, Ile
  • RNA, Transfer, Met
  • Electron Transport Complex I
  • MT-ND3 protein, human