Positive predictive value of Gen-Probe APTIMA Combo 2 testing for Neisseria gonorrhoeae in a population of women with low prevalence of N. gonorrhoeae infection

Clin Infect Dis. 2004 Nov 1;39(9):1387-90. doi: 10.1086/424880. Epub 2004 Oct 11.

Abstract

We collected specimens from women who tested positive for Neisseria gonorrhoeae using the Gen-Probe APTIMA Combo 2 (AC2), and we performed confirmatory tests using a nucleic acid amplification test (NAAT) that targets alternate gonococcal nucleic acid sequences. Among 59,664 specimens, 280 (0.47%) had positive results using AC2; 265 of these specimens were tested using the confirmatory NAAT, of which 258 yielded positive results (positive predictive value, 97.4%; 95% confidence interval, 95.1%-98.8%). Routine confirmatory testing of specimens with positive AC2 gonorrhea test results is not indicated.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Diagnostic Tests, Routine / methods*
  • Female
  • Gonorrhea / diagnosis*
  • Gonorrhea / epidemiology*
  • Gonorrhea / microbiology
  • Humans
  • Neisseria gonorrhoeae / genetics*
  • Neisseria gonorrhoeae / isolation & purification*
  • Nucleic Acid Amplification Techniques / methods*
  • Predictive Value of Tests
  • Prevalence
  • Sensitivity and Specificity