Abstract
Twenty-five Burkholderia cepacia-like isolates of human and environmental origin, comprising five different recA RFLP types, were examined by using a polyphasic taxonomic approach, including recA gene sequence analysis, 16S rRNA gene sequence analysis, DNA:DNA hybridisation studies, tDNA-PCR, fatty acid analysis and biochemical analysis. The results of the present study demonstrated that twenty-three of these strains belong to Burkholderia pyrrocinia, a B. cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques
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Burkholderia Infections / microbiology
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Burkholderia cepacia complex / chemistry
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Burkholderia cepacia complex / classification*
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Burkholderia cepacia complex / genetics
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Burkholderia cepacia complex / isolation & purification*
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Burkholderia cepacia complex / metabolism
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Cystic Fibrosis / complications
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Cystic Fibrosis / microbiology
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DNA Fingerprinting
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DNA, Bacterial / analysis
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / isolation & purification
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Fatty Acids / analysis
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Genes, Bacterial
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Genes, rRNA
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Humans
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Phylogeny
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 16S / genetics
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Rec A Recombinases / genetics
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Sequence Analysis, DNA
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Sequence Homology
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Soil Microbiology
Substances
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DNA, Bacterial
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DNA, Ribosomal
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Fatty Acids
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RNA, Ribosomal, 16S
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Rec A Recombinases
Associated data
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GENBANK/AF456045
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GENBANK/AF456049
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GENBANK/AJ440713
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GENBANK/AJ440714
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GENBANK/AJ440715
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GENBANK/AY099312
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GENBANK/AY099313
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GENBANK/AY099314
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GENBANK/AY321587
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GENBANK/AY392090