Distribution of heat shock protein 108 mRNA during the development of the chicken brain

Neurosci Lett. 2004 Nov 11;370(2-3):140-5. doi: 10.1016/j.neulet.2004.08.032.

Abstract

The developmental expression of heat shock protein 108 (HSP108) mRNA was mapped in chicken brain using in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR showed that HSP108 mRNA increased from embryonic day 5 (E5) to 13 (E13), significantly decreased from E17 to E21 and then increased again at the adult stage. In situ hybridization showed that while intense HSP108 positive (HSP108+) signals were localized in the cerebellum from E7 to E14, the intensities of these signals were significantly decreased at E17. However, at the adult stage, HSP108 expression increased in a cell type dependent manner. A decrease in HSP108 mRNA expression during differentiation was also observed in an in vitro study of brain cells treated with nerve growth factor (NGF).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Analysis of Variance
  • Animals
  • Blotting, Northern / methods
  • Brain / cytology
  • Brain / embryology
  • Brain / metabolism*
  • Cells, Cultured
  • Chick Embryo
  • Gene Expression Regulation, Developmental* / drug effects
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism*
  • In Situ Hybridization / methods
  • Nerve Growth Factor / pharmacology
  • Neurons / drug effects
  • Neurons / metabolism
  • RNA, Messenger / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Transferrin-Binding Proteins / genetics
  • Transferrin-Binding Proteins / metabolism*

Substances

  • Heat-Shock Proteins
  • RNA, Messenger
  • Transferrin-Binding Proteins
  • Nerve Growth Factor