Improving the expression of mini-proinsulin in Pichia pastoris

Biotechnol Lett. 2004 Aug;26(16):1269-72. doi: 10.1023/B:BILE.0000044871.80632.f9.

Abstract

Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 degrees C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l(-1) in the culture supernatant after 160 h of growth.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Ammonium Sulfate / pharmacology
  • Bioreactors / microbiology*
  • Cell Culture Techniques / methods*
  • Cell Proliferation / drug effects
  • Edetic Acid / pharmacology
  • Hydrogen-Ion Concentration
  • Nitrogen / metabolism*
  • Pichia / drug effects
  • Pichia / genetics
  • Pichia / growth & development*
  • Pichia / metabolism*
  • Proinsulin / biosynthesis*
  • Proinsulin / genetics
  • Recombinant Proteins / biosynthesis
  • Temperature

Substances

  • Recombinant Proteins
  • miniproinsulin
  • Proinsulin
  • Edetic Acid
  • Nitrogen
  • Ammonium Sulfate