The production processes of the petrochemical industry expose workers to high potential hazards. Our previous study showed that hazard recognition was closely related to worker safety and health training activities. The purpose of this study was to establish and validate a safety and health training model. It is expected that the training model will help workers to recognize hazards, thereby lowering their operating risks. The training model, which included a complete training course and follow-up scoring using a questionnaire, was applied to three groups of subjects for comparison. Group A had joined our study previously and took the training course again at this time. Group B had also joined our previous study but did not take this training course. Group C was new to our study and took this training course for the first time. Groups A and C (who took the training course) had higher cognition and attitude scores than group B (who did not take the training course). The training course was a significant factor that positively influenced both cognition and attitude scores among managers and workers. The training course was more significant for managers while the duration of education was more significant for workers.